Glucosamine Sulfate Inhibits TNF- and IFN- -Induced Production of ICAM-1 in Human Retinal Pigment Epithelial Cells In Vitro
نویسندگان
چکیده
METHODS. ARPE-19 cells were used as a model to determine the effects of GS on the expression of the ICAM-1 gene upregulated by TNFor IFN, by Western blot analysis and semiquantitative reverse transcription polymerase chain reaction (RT-PCR). The activation and nuclear translocation of the nuclear factors NFB and STAT1 were evaluated by immunocytochemistry, Western blot analysis, and electrophoretic mobility shift assay (EMSA).
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